Research: The nucleus and cytoplasm communicate continuously to sustain cellular functions. Our research asks a fundamental question: how is nucleocytoplasmic transport regulated in healthy cells, and how does it break down in diseases? We focus on the human nuclear pore complex (NPC), the sole gateway to nucleus, responsible for the selective transport of proteins and mRNAs through a ~50–60 nm channel. Addressing these questions requires observing transport events at the spatial and temporal scales at which they occur. While electron microscopy provides exquisite structural detail, it captures static states. In contrast, we directly visualize single cargo transport events in real-time 3D within transport active human NPCs. By combining biochemistry with cutting-edge 3D super-resolution microscopy, we try to uncover how this molecular gate operates and fails under cellular stress, viral infection and neurodegeneration. Our approach is simple: seeing is believing. If you’re excited to explore Nanoscale Biology, Super Resolution Microscopy, and the rapid dynamics of intracellular transport, join us to decode how the nuclear gate functions in health and disease. Curious to learn more? Explore the recent ‘Publications’.